I am a bit confused about the matter. The majority of papers and explanations I have come across indicate that the inhibited line is generally positioned above the uninhibited line, but I have observed it to be the opposite. will it still be considered as lineweaver burk plot?
Since a L-B plot is of the reciprocals of the rates and substrate concentrations, a decrease in activity caused by the presence of an inhibitor is represented as a line that is higher up in the first quadrant than the uninhibited line. If you are seeing the opposite effect, it means that the enzyme was more active in the presence of the inhibitor than in its absence. In other words, the compound is an activator, not an inhibitor. However, it would be a good idea to share the data, since there may be other interpretations that can only be made based on seeing the data.
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