Maryam Ansari

6 Questions 7 Answers 0 Followers

Questions related from Maryam Ansari

Why does the level of expression of target gene in negative control group(scramble si RNA)is lower than the non treated group?

I used both lipofectamine RNA iMax and electroporation method and always gene expression level in negative control group is 40% lower than untreated group.

08 October 2024 2,512 3 View

Cut PDMS sheet with Graphtec cutter plotter CE6000-40?

Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?

26 June 2024 8,377 1 View

Dear All, Is any body have experience how to use the TriFECTa RNAi kit for transfection?

I want to use the TriFECTa RNAi kit for transfection, but I dont know how much RNA and control transfection should I use.

13 March 2024 1,647 2 View

Dear All, Could we auto-cleave sigmacote?

Is it changed it 3D structure and damage the structure or not?

22 January 2024 2,284 1 View

How could I culture HUVEC cells in a microfluidic device and after 24 hour they cover all the surfaces?

At first, when I culture cells I could obviously see cells in channels that moving through the channels, but after 24 hour there is no cell.

29 November 2023 6,970 7 View

Increasing hydrophilicity of PDMS

What is the most efficient time to oxygen plasma of PDMS to increase the hydrophilicity?

01 January 1970 2,836 2 View