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Questions related from Saranpal Chhabra
I recently performed H&E staining on my paraffin embedded P0 mice tissues to visualize the phenotype of the eye in one of my WT mice. I managed to slice the tissues and stained them with...
05 May 2017 9,927 4 View
Hi, I have got CD results for my protein. However I do not know how to interpret the data for CD. I have uploaded my results in word format. Kindly download to take a look at the results. I...
06 June 2015 3,683 9 View
Dear Esteemed Researchers, I am trying to solubilize a membrane protein that forms inclusion body when expressed heterologously in E. coli cells. As such, I tried various detergents to screen the...
05 May 2015 1,488 14 View
Hi all, We have HiLoad Superdex 200 16/60 Gel Filtration column in our lab, which have some 'Sticky Proteins' and protein aggregates bound to the column. As such, I wish to perform some rigorous...
12 December 2014 2,507 9 View
I realized that this often happens only when I perform purification. My protein is fused to a 60kDa tag and my protein it self is ~20kDa. So taken together, the fusion protein should result in...
08 August 2014 5,372 23 View
I have recently extracted total RNA from the bark of Rubber tree. However, I am not sure if these RNA's are contaminated with gDNA? I see some smears on the RNA. Could the smears represent gDNA?
07 July 2014 8,735 7 View
RG has become the benchmark and perhaps the most famous social networking platform for many of the researchers/scientists/students etc. As such, do you think it would be appropriate if one were to...
04 April 2014 6,248 5 View
I have recently performed cleavage of my fusion protein using Thrombin (Image as attached). However, after running the thrombin treated samples with a control (untreated with thrombin), I could...
03 March 2014 3,382 2 View
I have recently extracted total RNA from a plant. Upon extracting, I QC checked the RNA via NanoDrop, gel electrophoresis and Bioanalyzer. The NanoDrop reading showed good RNA concentration as...
03 March 2014 3,112 5 View
Can Protein be quantified using NaNoDrop? Using the value of the Ext. Coefficient and MW of my protein that I obtained from Expassy ProtParam, I quantified my protein using NanoDrop. I then also...
02 February 2014 2,022 15 View
I have performed transformation of my gene into Pichia to express my desired recombinant protein. Now that I have successfully transformed, I would like to know if my gene has been integrated into...
01 January 2014 6,159 3 View
I will be performing electroporation on Pichia to express a recombinant protein. Is it possible to use 0.1cm cuvette (Frequently used for E.coli or other bacteria) rather than 0.2 cm cuvette? Will...
12 December 2013 6,482 10 View
I have cloned my gene downstream of the NusA tag using BamHI and HindIII as my restriction sites. Now that my protein is expressing, I would like to cleave this tag and purify the protein to...
11 November 2013 2,740 1 View
Toxic Protein?
08 August 2013 3,471 2 View
Suppose that I am using 1kbp DNA marker
07 July 2013 9,579 23 View
I have performed cloning of my eukaryote (plant) gene into pET21a vector. Though, the vector contains His-tag at its C-terminal, the primer of my gene of interest does not carry sequence for the...
07 July 2013 2,363 14 View
