Yes it does, even for preparing media for tissue culture, a decimal digit difference is often kept as a margin when steralization is done using filter emmbranes.
Water comes first from the membrane as it has the minimal molecular size thus, initially the solution becomes near neutrility first and then shifts to respective pH but it never reaches to the same as unfiltered one as some of teh left over also trapps some hydronium ions with it as for its own aqueous ionization.
Yes it does, even for preparing media for tissue culture, a decimal digit difference is often kept as a margin when steralization is done using filter emmbranes.
Water comes first from the membrane as it has the minimal molecular size thus, initially the solution becomes near neutrility first and then shifts to respective pH but it never reaches to the same as unfiltered one as some of teh left over also trapps some hydronium ions with it as for its own aqueous ionization.
The only thing I can think of is that during the filtration the sample is degassed, changing the level of carbonic acid. Does the pH return to the original 7.4 after exposure to air after a few hours? I've only observed this with unbuffered solutions though...
DPBS is not a very strong buffer, i.e. the ionic strength is rather small. Therefore, it could be that leachables from the membrane interfere with the buffer and thus, change the pH. My recommendation would be to wash the filter with WFI prior to pass the buffer through. Alternatively, the filter could be washed with a part of the buffer (50-100 ml) first which is discarded. Then the rest of the buffer is filtered. This can easily be checked the next time when you prepare a buffer.
The Donnan effect is not applicable I think because the filter is not a semi-permeable membrane.