I kept adherent cells at 37C vs 4C after formalin fixation and over a few days, the cells uplifted at 37C but remained attached to the plate at 4C. I can't find any mechanisms or literature to support this finding, does anyone know why?
If tissue was fixed in 4% PFA then cryoprotected in 30% sucrose, are you able to wash and switch to FFPE processing instead of blocking frozen in OCT?
03 October 2023 198 3 View
Are there phenotypic and functional differences in DCs generated from culturing BMDCs (mouse) in the presence of GM-CSF vs. FLT3L. Also does using human vs. mouse FLT3L make a difference? Many...
03 August 2023 877 1 View
Product of interest: ZeptoMetrix Norovirus GI and GII External Run Controls. https://www.fishersci.com/shop/products/nattrol-gp-external-run-controls/21100555 I am trying to run the RNA...
07 May 2023 3,968 3 View
I have a mixture of goat serum / plasma that I attempted to ammonium sulfate precipitate (using pooled samples) to get out the immunoglobulins. I dialyzed in PBS afterwards, but when placed in the...
29 March 2023 3,791 1 View
Is it possible to obtain readable results using a loading control conjugated antibody (e.g., Rb anti-Gapdh 680) along with a target protein unconjugated antibody plus secondary, if the target...
14 March 2023 2,821 2 View
I am trying to grow spirulina at home but i am a bit confuse. Now every time I turn the air pump on the water starts to create some bubbles so I can't turn it on because it will spill all of my...
17 February 2023 5,461 1 View
Finding the links between behavior and online multiplayer gaming.
03 February 2023 4,727 0 View
Hello, I am currently working with serum samples to perform a Western Blot to identify a series of target proteins. I am having trouble calculating the amount of sample I need to load. I would...
28 January 2023 9,654 1 View
I am working on a mycoremediation project and trying to sperate the mycelium from the soil it is growing in to test for pollutants. Just looking for some new ideas.
17 January 2023 6,876 2 View
as an educator in conservation, it is very important that i teach methods that are non destructive. such as counting insect visitation to flowers, for pollinator diversity and abundance studies. i...
07 December 2022 4,003 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View