Megan DeZago

3 Questions 5 Answers 0 Followers

Questions related from Megan DeZago

Why does storage of fixed cell samples at 37C cause cells to uplift?

I kept adherent cells at 37C vs 4C after formalin fixation and over a few days, the cells uplifted at 37C but remained attached to the plate at 4C. I can't find any mechanisms or literature to...

18 May 2020 5,266 0 View

How to do aptamer SELEX with protein target on magnetic beads?

I have a His-tagged recombinant protein that I'm attaching to Dynabeads for aptamer targeting. Once the aptamer library binds, how do I remove the aptamers from the protein for amplification? I've...

03 June 2019 8,445 4 View

Protocols for getting started with aptamers?

I'm trying to do some proof of concept aptamer generation for proteins (like thrombin or insulin, things with published aptamer sequences to comparison). I'm trying to use the most standard...

22 January 2018 8,664 2 View