In preformulation process , assessing deamidation should be done in short term. In deamdation reactions ( we have fiffernt reactions), Asn usually is the main residue to be converted. Does Asn have a critical role in the stability of proteins' structure. I know about its role in turns and pseudo turns. Are these roles critical or with changing Asn the rate of immunogenicity increase. I could not find any source about how deamidation changes the stability. Thank you in advance for any commnet.
In addition to immunogenicity, it may modulate the potency in some cases.
These articles can help you:
http://www.ncbi.nlm.nih.gov/pubmed/23115046
http://www.ncbi.nlm.nih.gov/pubmed/16960822
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104603/
From a biophysical viewpoint, deamidation increases the net negative charge of proteins and can destabilize them by charge repulsion effects.
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