This is a 1% agarose gel in 1xTBE with 1xTBE running buffer, visualized on UV transilluminator using ethidium bromide. The markers are 1KB+, and the rest of the signals are my plasmid miniprep samples, isolated using ethanol precipitation, after a diagnostic digestion, loaded with common ficoll loading buffer with bormophenol blue and zylene cyanol. Apparently, there's something in the digested samples migrating near the lower marker bands that causes the markers to constrict. When I use TAE I don't see this. (Sometimes I use TBE for better resolution of smaller bands.) Any ideas?
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