Hi Khawla,

Several studies have been conducted on plants as a source of anti-inflammatory activity. However, the parts of the plants used and the compounds responsible for the anti-inflammatory activity have not yet been fully elucidated, I would also like to point out that IC50 (antioxidant activity) is inversely related to the polyphenols content. May I be informed of the botanical/family name of the plant you are working on? this will allow refining the search a little bit. Regards

thank you very much Abderrahim Alahyane for your answer. regarding the DPPH assay, the leaves showed a low IC50 which wich means a higher antioxidant activity compared to the stems. the plant studied belongs to the Euphorbiacea family.

I just can't justify this difference in anti-inflammatory activity.

Thanks in advance

You can discuss the result that the stems maybe contain phenolic compunds that are different from the phenolic compounds in the leaves and have more anti inflammatory activity. it is not just about concentration of phenolic compounds but also about the type of them because there are Structure - activity relationship between phenolic compounds and their anti-inflammatory activity.

in my research I used the inhibition of albumin method and the stability of RBC membrane method to determine the anti inflammatory activity of phenolic compounds in fruit juices and as you can observe that all juice samples had higher percentage inhibition at low phenolic concentrations and there are many studies like my results.

Article In-vitro Anti-inflammatory activity of Total Phenolic conten...

you can check my article and see the articles in the references too .

Good luck Khawla Bouaouda

The simple reason is that the phytochemicals responsible for the anti-inflammatory activity are more abundant in the stem, or the stem may contain secondary metabolites different from those contained in the Leaves. Different plant parts are known to contain different kinds or different proportion of phytochemicals. I will be necessary to isolate the compounds from both parts and study them

Thank you very much Akram Nezam and Fabian Ifeanyi Eze

Well, phenolic amounts are usually associated with antioxidant activity of plants.

Now, the leaves containing higher phenols justifies its higher antioxidant activity than stem.

In addition to this, the stem maybe abundant in another phytochemical which you ought to determine and conclude your work. Because some other phytochemicals are also known for anti-inflammatory effects too.

The distribution of secondary metabolites in plants depends on adaptation, function, time (season) and structural features of the metabolite. E.g., aromatic and color-imparting flavonoids are more concentrated in the leave and flowers of plant than the stem and root due to the role they play in say; pollination, photosynthesis, etc. On the other hand, heavy metabolites are more abundant in the roots and stem than in the leaves. It's not a general rule that the stem is richer in anti-inflammatory metabolites than the leave. It can vary from plant to plant depending on the aforementioned factors.