Rich C Joseph

5 Questions 19 Answers 0 Followers

Questions related from Rich C Joseph

What are the most reliable mathematical models for determining IC₅₀ in antioxidant assays, and how do they compare in accuracy and reproducibility?

IC₅₀ (half-maximal inhibitory concentration) is a crucial parameter in antioxidant studies, representing the concentration of a substance required to inhibit 50% of free radicals (e.g., DPPH,...

02 April 2025 2,124 2 View

In column chromatography, is it better to add powdered plant sample directly or use an extract, possibly dried with silica gel, for better separation?

Column chromatography is widely used for fractionation of plant extracts. I need the best approach for optimal separation efficiency

02 April 2025 935 1 View

Would I get an effective result if I use my extract contains the solvent used for extraction?

For e.g in antimicrobial studies, I want to carry out an assay using "Methanolic extract" of a plant sample. Is it advisable since methanol as a solvent possess some antimicrobial properties? Will...

26 March 2023 5,815 6 View

Can someone explain why "a plant extract mixed with a soln of AgNPs could change color even without heating for a period of time" during synthesis?

This is question is based on Green synthesis of plant materials using Silver Nitrate

26 March 2023 6,630 2 View

How do we really interpret some IC50 results?

I noticed in a particular result, the higher the concentration, the percentage inhibition was equally going higher. But there is an issue, when the IC50 was calculated using Linear regression,...

12 April 2022 5,191 3 View