Can you help me to provide standard curve of NH4Cl? i want to quantify the adsorbed quantity of NH4Cl and first i should provide standard curve of NH4Cl. So i tried to draw the calibration curve with 5mg/L, 20mg/L, 50mg/L, 100mg/L, 250mg/L and 500mg/L*3, but i thinK something is wrong in my work because the absorbance is very low
Thank you for any information33.
Absorbance in which wavelength? And could you show the regression you obtained?
Hello,
Here is some of the similar topics:
https://www.researchgate.net/post/How_should_I_prepare_the_standard_curve_for_soil_urease_activity
https://www.researchgate.net/post/How-can-i-provide-standard-curve-of-NH4Cl-for-urease-assay
I wish you find it helpful
Jokin Ezenarro This morning I tried to work with small concentrations but the calibration curve did not give good results. In fact, as the concentration increases, the absorbance decreases and there are no great differences between the values. What's more, I found the wavelength to be around 360 nm, which is different from what I've seen in the literature (530-640).
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