we extract RNA and run on 1.2% agarose gel but could not see the distinct band of mRNA on the RNA gel.
total rna contains 80% of rRNA and only 3% of mRNA. That is why it is difficult to see it in gel due to the lower percentage and thats why we analyse the RNA integrity by looking at the three rRNA bands. Also, every gene forms a different sized mRNA, so the mRNA will be present in different bands all over the lane in the gel but you cannot see it.
Did you mean "distinct" or "distant"? If you see a fuzzy trail this means your RNA has degraded. What is the source and how do you extract it? I would revise the protocol if it degrades again.
sorry i mean distinct, i extracted RNA from barley, I want to know whether we can see mRNA on the gel or not.
total rna contains 80% of rRNA and only 3% of mRNA. That is why it is difficult to see it in gel due to the lower percentage and thats why we analyse the RNA integrity by looking at the three rRNA bands. Also, every gene forms a different sized mRNA, so the mRNA will be present in different bands all over the lane in the gel but you cannot see it.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA