I used as ypotonic solution 10 ml KCL 0,056 M for 30 min.
i let the cuture with fix in fridge for 40 minutes and i make only one wash with fix. finally i resuspend the pellet in 2ml FIX. Any ideas?
Hi Katerina, I don't know the specifics of your procedure, but K+ can form insoluble salts with anionic detergents such as SDS, cholate, etc.
What is the composition of your fix?
I use (3methanol:1acetic acid ) as a fixative
KCL is sparsely soluble in organic solvents compared to H2O. Can you switch the fixative to 1% paraformaldehyde in PBS and see if you get the same crystals?
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