Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Do I have the right PCR cycler settings?

Hello. Someone designed primers and cycler settings for me and it doesn't work. I do hot start PCR with touchdown, I detect Babesia sp. DNA in Acari. The primers are F: GYYTTGTAATTGGAATGATGG, R:...

01 March 2020 3,949 3 View

How do I determine differences between two hemp seed samples to determine if they are genetically different strains or the same strain?

Are there currentlly any methods developed for determining the differences in genome between two samples?

08 August 2019 4,594 2 View

My cells started to form clumps during passaging. I tried almost everything to reduce them. Do you have any suggestions how to solve this problem?

My cell cultures (HeLa S3, mouse kidney cells, WB-neo, WB-ras) strarted to form clumps during passaging so it is not possible to count them properly. I TRIED 1. make PBS without Mg and Ca...

14 January 2019 9,398 6 View

How to remove the Percoll from the sample?

I use density gradient centrifugation with Percoll to extract bacteria from the bentonite sample. It is working quite well, but I need to remove the Percoll from the bacterial extract at the end...

25 July 2018 981 3 View

How influence the concentration of oxygen growth of oxide layer and properties of oxide layer itself due to thermal oxidation?

Hello, I'm working on my diploma work on thermal oxidation of TiNb alloy.In my work I have oxidized titanium by two procedures thermal oxidation in laboratory atmosphere and in atmosphere of...

22 May 2018 1,704 5 View

F.I.S.H probe: Can be used as a probe a pcr labeled product with 600-1000 bps size?

Is it nessessary to be inserted in a BAC clone?

05 June 2016 3,512 1 View

I'm looking for taxonomic keys (for genera) - for identification of grasshoppers from Borneo (Malaysian part). Please, do you know some keys?

01 March 2016 6,885 5 View

Does anyone know whether a chromatography mode exists when both the stationary and mobile phase (developing solvent in TLC) are nonpolar?

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10 October 2012 8,485 11 View

Stimulation of CD8+ CTL with specific antigen in vitro

How to increase effectivity of CTL stimulation with synthetic peptide epitopes.

13 October 2011 2,226 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View