Katerina Pantopikou

5 Questions 6 Answers 0 Followers

Questions related from Katerina Pantopikou

F.I.S.H probe: Can be used as a probe a pcr labeled product with 600-1000 bps size?

Is it nessessary to be inserted in a BAC clone?

06 June 2016 3,658 1 View

Why could I have in mFISH chromosomes with almost the same colour?

the colours that paint almost all my chromosomes are violet blue and green. Is there any problem with the procedure or something wronge with the programm-microscope settings? Moreover when i want...

01 October 2015 5,344 4 View

Why i cant find my metaphases after m fish preparation ?

i prepare slides with typical cytogenetic precedures. i find lots of good metaphases in the microscope and then i start the mISH experiment. But when i want to focus to the visible light in the...

01 September 2015 7,008 12 View

How should I diulte trypsin in PBS for g banding?

how much trypsin should i dilute in PBS for g banding? i also cant understand if i let my slide to much time in trypsin or not. Can anyone show me a picture of overtrypsinised chromosomes and...

14 August 2015 421 4 View

Why do I observe crystals after metaphase preparation in blood sample?

I used as ypotonic solution 10 ml KCL 0,056 M for 30 min. i let the cuture with fix in fridge for 40 minutes and i make only one wash with fix. finally i resuspend the pellet in 2ml FIX. Any ideas?

20 July 2015 5,726 3 View