I am looking at the expression of a particular protein in the skin by immunofluorescence and I would like to know which cells express this protein.
For that, I stained both cryosections and formalin-fixed skin sections and I obtain different kind of staining depending on the type of sections, the protein seems to be expressed by different cell types/compartment. Could the same antigen be detectable in cryosections but not in paraffin sections ? I tried 3 different antibodies and I always have the same issue, the staining in cryosection is not similar as the staining in paraffin sections.
Thanks for your help.
Well if you have a different fixation method (cryo or formalin) you might consider a different antigen retrieval (heat vs. enzyme etc), which might make the cells more susceptible for the antibody.
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