How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Seeds of wild Brassica rapa?

Hi, We are a team working on wild and cultivated plants and our stock miss of seeds of Brassica rapa from wild populations in Europe. We are based in Finland, we wonder if any laboratories in...

25 October 2020 857 3 View

Which proteins are continuously expressed (at stable levels) in mammalian monocytes and thus, suitable controls for Protein expression studies?

Hello! Currently I am testing whether an externally added agent affects the expression of only one particular protein or whether it generally inhibits protein synthesis due to its toxicity. For...

14 September 2020 6,359 3 View

Suggestions for an easy experiment on an inverted fluorescence microscope (students lab course)?

09 September 2020 290 3 View

Why it is that Disulfiram (DSF) only binds to zinc in cancer cells?

Good Afternoon, I understand that the zinc transporter proteins (ZIP7 and ZIP10) are both present in healthy and diseased cells. I learnt that zinc uptake is elevated in malignant cells...

09 October 2019 5,069 3 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Compare multiple means over time, which analysis?

I have a repeated measures design, looking at how sex hormones effect number of responses on a dichotic listening task, three times over the course of the menstrual cycle. Where it simply the...

14 April 2019 8,108 3 View

What is the best way to extract microRNA in postprandial blood?

Because of lipids in postprandial blood, I have some troubles to extract microRNA. How can I remove lipids without alter the quality of microRNA?

25 February 2019 2,781 3 View

What is the best way to know the quality of RNAm from plasma?

Hi everybody, I did extraction of total RNA on plasma samples (I am interested in circulating microRNA). How can I do to know if my sample are of good quality and if there are no...

01 October 2018 4,786 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How I can add publication with maiden name?

Good morning, can You please tell me how I can add an article that was published under my maiden name? When I try to add an article with this name, it appears information that I should be an...

21 February 2021 6,866 3 View