What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

How can suspending cell lines (e.g. THP-1) not attach the culture flask bottom?

As the cell density usually higher than culture medium, I just wonder how can suspending cell lines won't attach the flask bottom?

03 August 2020 9,892 5 View

How to simulate the EQE by SCAPS?

Hello, I simulated solar cell according the experiment, Voc,Jsc,FF and PCE is fine. but the EQE is very different compared with experiment. I don't know how to simulate it? I don't consider the...

02 August 2020 1,236 3 View

How can I detect the presence of antibodies in plasma using electrophoresis?

Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...

30 July 2020 3,400 1 View

How to simulate the end-to-end delay of mobile network with MATLAB?

I want to simulate the end-to-end delay of a packet from the source to the terminal with MATLAB, and it is better to use 5g mobile communication network.Please help,thanks.

05 July 2020 6,027 4 View

How Can I Detect if the cultured cells are lysed ?

I want to prove a kind of protein in medium is from exocytosis but not lysed dead cells. I can detect the protein in medium by ELISA(no band in Western blotting), but I'm not sure where does the...

20 January 2020 8,768 4 View

Does anyone know which company's Flag antibody from rabbit source can work well in immunofluorescence?

Does anyone know which company's Flag antibody from rabbit source can work well in immunofluorescence?

19 July 2019 7,658 4 View

Why precoating Pluronic F127 can inhibit cell adhesion on PDMS surface? Any technical articles recommend?

It is known that coating pluronic F127 inhibit cell adhesion on PDMS or glass. I just curis about the mechanism of the suppression. Thanks in advance!

06 June 2019 6,619 1 View

What is the meaning of gm in Cadence?

I builded a circuit to simulate NOMS' gm, and found it 16.29 mS. But if I use the formulation learned from class, gm=2*Ids/(vgs-vth). Its result is 27.7 mS. I guess the gm in Cadence is...

10 November 2018 732 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View