NO
Maybe you could try nanoindentation to get the modulus of elasticity for conversion
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?
20 May 2024 8,214 2 View
The development of mRNA vaccines on mice and rabbits?
18 May 2024 7,475 2 View
We have constructed lentivirus transduced U251 cell lines to express (1) EGFRvIII (its amino acid sequence is MRPSGTAGAAFLALLAALCPASRALEEKKGNYVVTDHG..., which is identical to the sequence...
14 April 2024 2,370 1 View
It is a common belief that extreme weather and climate events increased in frequency and intensity over the last century or half a century, a time period that saw a rapid global climate warming....
16 February 2024 3,727 10 View
Does anyone know where I can find the data of measured above-ground biomass? I need the data of forest biomass measured on the spot for training and learning. If possible, I can also invest money...
12 November 2023 7,845 3 View
How can we achieve the effect of the added magnetic field on the electrolysis process during molten salt electrolysis?
01 September 2023 4,416 2 View
I'd like to know what's current progress of 'synthetic circadian clock' or 'synthetic oscillators'. Indeed, I would like to investigate methods to regulate protein expression (e.g., transcritpion)...
24 August 2023 8,165 1 View
Weather stations are key facilities to record long-term change in surface air temperature (SAT) in land. However, some weather stations are located in or near cities, and they may suffer from...
20 June 2023 8,860 2 View
Can anyone suggest a good method for the preparation of naphthaldehyde dimethyl acetal? I have been looking for chemical vendors that might be selling it but couldn’t find one. Other methods I...
14 June 2023 505 0 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
How do you tie-break and rank these two in their strength?
03 August 2024 4,471 1 View
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
