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Maybe you could try nanoindentation to get the modulus of elasticity for conversion
I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...
21 September 2020 4,321 2 View
Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...
24 August 2020 2,352 1 View
As the cell density usually higher than culture medium, I just wonder how can suspending cell lines won't attach the flask bottom?
03 August 2020 9,892 5 View
Hello, I simulated solar cell according the experiment, Voc,Jsc,FF and PCE is fine. but the EQE is very different compared with experiment. I don't know how to simulate it? I don't consider the...
02 August 2020 1,236 3 View
Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...
30 July 2020 3,400 1 View
I want to simulate the end-to-end delay of a packet from the source to the terminal with MATLAB, and it is better to use 5g mobile communication network.Please help,thanks.
05 July 2020 6,027 4 View
Dear research fellows, I encountered a problem recently and don't know how to explain. I reduced the flow rate in my HPLC method by 25%, the areas in my stds increased by 25% as expected, however...
16 May 2020 1,696 6 View
I want to prove a kind of protein in medium is from exocytosis but not lysed dead cells. I can detect the protein in medium by ELISA(no band in Western blotting), but I'm not sure where does the...
20 January 2020 8,768 4 View
Does anyone know which company's Flag antibody from rabbit source can work well in immunofluorescence?
19 July 2019 7,658 4 View
It is known that coating pluronic F127 inhibit cell adhesion on PDMS or glass. I just curis about the mechanism of the suppression. Thanks in advance!
06 June 2019 6,619 1 View
I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...
01 March 2021 6,661 3 View
I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...
27 February 2021 6,801 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!
25 February 2021 3,092 2 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
