Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?
you may plate the hybrids even without Feeder layer in HAT selection medium (Sigma-Hybrimax). The dead cells will eventually be cleared during medium change every third day.
Hi
I guess this product may help you. I never try it myself, but I guess you could find more information from stemcells.
https://www.stemcell.com/clonacell-hy-medium-d-without-hat.html
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