Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.
Phenol red containing formulations are manufactured with DMEM containing phenol red. The color variations that are observed in frozen & thawed that contain phenol red may range from straw yellow to dark red. This is due to the interaction of carbon dioxide with the bicarbonate buffer and the phenol red. The color variation does not affect product efficacy, and will disappear upon equilibration with 5% CO2.