Why did diiferent amounts of sample give same absorbance in mcp1 elisa kit (sandwich)?

More Aseel Shakhatreh's questions See All

How can I quantify confocal images using JACOP? which analysis can I choose and based on what?

Hi, I am currently running an experiment to determine the internalization of LDL with LDLR. I need to quantify my images. It's a bit difficult to quantify Dil-LDL since Dil-LDL appears as single...

03 October 2023 5,864 0 View

How can I filter a dissolved modified citrus pectin (MCP) ?

Filtration of MCP

09 May 2023 6,380 2 View

What is the best intrapertonneal injection protocol for LPS (Sprague dawelly rats) that will be enough for induction of cytokines elevationc in GI?

I have searched in a litrerure for a protocol to induce stress and inflammation with LPS in Sprague dawely rats in colonic and intestinal tissues( , but l mostly eaither found protocols for...

04 March 2023 2,020 1 View

How could I evaluate the efficacy of LPS that I have ?

TNF alpha, IL-6, and IL-1b protein expression has not elevated after the colon and small intestine tissues(.5cm ) were treated with one microgram/ml for tissue incubation with DMEM media for 6...

07 January 2023 4,542 4 View

This is a part of carbon surface modification with gold NP, could any one help to know what is suitable wavelength for the used protocol ?

Hello, I am doing a carbon surface modification with Gold nanoparticles. from the FTIR below, I know the formation bond of NH3 and for COO , but I would like to know what is the range of thiol...

20 September 2021 525 4 View

How can I dissolve anthracene and prepare the dilutions without using Ethanol or any type pf alcohols or with a tiny amount of ethanol not exceed 1%?

I am trying to test Anthracene (An) on the cells and I could not dissolve An in buffer solution and even in ethanol the solubility is very poor. DMSO is not working as well! for my experiment I...

27 May 2021 8,350 1 View

Do anyone have any idea if i can use VEGFR inhibitor in vitro on cancer cell lines ?

How can i know the expression of VEGFR on certain cell line ? any recommendation !

24 June 2020 6,479 4 View

FACS Buffer solution, FBS content?

First Q: Most protocols allow for 1-5% FBS in FACS buffer solution, however, I use 10% FBS and I never had problems with the sort. what is the reason behind the reduced FBS? Second Q: Is it okay...

22 April 2020 9,357 5 View

Phosphorylated protein storage?

My question is about the storage of proteins (to probe for phosphorpoteins) after adding SDS buffer and B-meracptoethanol, reducing for 5 minutes at 95c, is the sample going to be stable at -20C?...

01 August 2019 3,217 4 View

I got a liquid DNA primer! does anyone worked with liquid primers before? and it came without colling! no dry ice , nothing?

Infos I have on the sheet is 23.3 nmoles, OD 5.20. The question is, is it already 100mM or do I need to dilute? No idea! Please let me know if you have experience with this!

19 June 2018 4,413 4 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View

VECM Model: Positive/insignificant CointEq1 ?

Hello everyone, I am conducting

22 July 2024 8,085 11 View

Merge Intensity Data from 2 runs?

Hi, We have collected several images for two independent runs of an experiment using two different confocal microscopes and settings. Thus, the intensity values collected through ImageJ were...

22 July 2024 8,722 0 View

What is the solvent system required to run tlc for calixarenes?

If you don't understand my question let me know

21 July 2024 563 1 View