Aseel Shakhatreh

3 Questions 4 Answers 0 Followers

Questions related from Aseel Shakhatreh

What is the best intrapertonneal injection protocol for LPS (Sprague dawelly rats) that will be enough for induction of cytokines elevationc in GI?

I have searched in a litrerure for a protocol to induce stress and inflammation with LPS in Sprague dawely rats in colonic and intestinal tissues( , but l mostly eaither found protocols for...

05 March 2023 2,021 1 View

Why did diiferent amounts of sample give same absorbance in mcp1 elisa kit (sandwich)?

I did optimization for ElISA (sandwich) for MCP-1 CHEMOKINE I did running for standards and blank I also performed running for one sample with different 5 amounts? All were out of the range and...

10 January 2023 3,100 2 View

How could I evaluate the efficacy of LPS that I have ?

TNF alpha, IL-6, and IL-1b protein expression has not elevated after the colon and small intestine tissues(.5cm ) were treated with one microgram/ml for tissue incubation with DMEM media for 6...

08 January 2023 4,543 4 View