Hi,

I am currently running an experiment to determine the internalization of LDL with LDLR. I need to quantify my images. It's a bit difficult to quantify Dil-LDL since Dil-LDL appears as single dots or accumalated dots in the merged image attached. I read about colocalization analysis using JACoP plug in on image j but I am quite lost between which analysis should I choose? whats the difference between Pearsons, Manders, Costes etc..

How can I quantify this and how many cells should I have per image?

Thank you in advance.

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