Hey friends, i am a bit curious about to know, why host strain (e.g. E. coli) changes its colour (pale yellow) to white after cloning..!!
Ammonium sulfate Vs TCA Vs Acetone?
01 February 2015 6,763 3 View
Hey friends, I need your help. I am trying to express a probable cellulase genes (1.2 kb) in pET28a vector (Novagen). I followed the protocol as per kit. But i didn't find the colonies (Novablue)...
01 February 2015 5,598 20 View
01 February 2015 3,535 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....
21 February 2021 8,609 1 View
What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...
21 February 2021 1,831 4 View
I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...
18 February 2021 8,198 6 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...
17 February 2021 4,118 1 View