Yogesh Chaudhari

4 Questions 47 Answers 0 Followers

Questions related from Yogesh Chaudhari

Why colony changes its colour after cloning..??

Hey friends, i am a bit curious about to know, why host strain (e.g. E. coli) changes its colour (pale yellow) to white after cloning..!!

03 March 2015 297 0 View

What is the most popular method for cellulase purification?

Ammonium sulfate Vs TCA Vs Acetone?

02 February 2015 6,854 3 View

Cellulase assay: Congo red Vs Grams iodine?

For the isolation and screening for cellulase positive microorganisms, which stain works well, congo red or grams iodine?

02 February 2015 3,620 5 View

No colonies in the pET28a cloning with experimental DNA (Cellulase gene-1.2kb) ?

Hey friends, I need your help. I am trying to express a probable cellulase genes (1.2 kb) in pET28a vector (Novagen). I followed the protocol as per kit. But i didn't find the colonies (Novablue)...

02 February 2015 5,699 20 View