Hello everyone.

I run a SDS PAGE on wheat anthers for my fist time, and I have got this outcome (see annex). In the first line (left to right), I loaded 10ul of ladder ( Spectra Multicolor Broad range Protein Marker - Thermo Scientific), second line it was 10ul of FANCM protein control (quite old) and the following lines I loaded 10ul of my samples (6-12 anthers of different sizes each sample). I stained in Coomassie R250 for 1h and then I destained for 1h. After that, I only see one thin and diffuse band in my samples, and the ladder doesn't seem defined. Does anyone have any clue about such issue?

Thank you very much.

Looking forward to hearing from you,

Chiara

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