Muhammed Zidan Khan

10 Questions 6 Answers 0 Followers

Questions related from Muhammed Zidan Khan

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

15 July 2024 6,674 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

07 July 2024 8,337 2 View

Why are the mice in my control group mildly hyperglycaemic?

I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a...

09 May 2024 3,820 2 View

What are alternative methods to ELISA analysis of serum and tissue homogenates for the quantification biomarkers?

I am working with C57BL/6 mice for the development of a T2DM mouse model. During the course of this experiment, the mice will have their urine, blood, and tissue samples collected and examined for...

17 April 2024 502 2 View

What are some good in-silico methods for pathways, biomarkers, and interactions that present in diabetic microvascular complications?

The four diabetic microvascular complications I am assessing are nephropathy, neuropathy, cardiomyopathy, and retinopathy. Are there any in silico methods which can be employed to identify...

17 April 2024 1,297 0 View

Can you perform fasting blood glucose tests on mice weekly?

My study focuses on developing a mouse model for the type II diabetes, narrowing in on a condition resembling chronic, late-stage diabetes. As such, blood glucose levels would need to be measured...

24 October 2023 8,082 4 View

How to validate the development of diabetic microvascular complications in a STZ-induced mouse model?

My project aims at producing a reproducible mouse model for T2DM and its related microvascular complications to aid in studying their interrelation, and combination therapy. The disease will be...

25 September 2023 2,348 0 View

How can I calculate the number of animals and groups I need in animal research?

My study aims at developing an animal model for type II diabetes. I don’t quite understand the power calculation, particularly in regards to the values I would substitute into the equation, as it...

24 September 2023 402 3 View

How to choose STZ dosage groups for chemically-induced diabetes in mice?

My research aims to produce a novel mouse model for T2DM and I have two dosage techniques to administer STZ in C57BL/6 mice: single high dose (SHD) and multiple low dose (MLD). Within these...

12 September 2023 637 3 View

What is the difference between streptozotocin dosage for type 2 diabetes in rats as compared with mice?

I am trying to understand the disparity in dosage of the beta-cell cytotoxic agent streptozotocin, specifically for the induction of type 2 diabetes, between rat models and mouse models. We are...

20 March 2023 586 5 View