I am trying to understand the disparity in dosage of the beta-cell cytotoxic agent streptozotocin, specifically for the induction of type 2 diabetes, between rat models and mouse models. We are attempting to induce t2d in mice, while most of the studies online document rats as their model of choice. The goal is sustained hyperglycaemia, while accelerating the diabetic condition and concurrently preventing animal death by cytotoxic effects, at least for a 20 week period (although a longer period would be preferred). Please help.
Dear Muhammed Zidan Khan
Although my research direction is not quite the same as yours, I have used db/db mice to induce diabetes type II as model animals in my experiments, and they maintained stable Hyperglycaemia from the beginning to the end of the investigation for 21 days.
The following is my research for your reference.
Article The Antimicrobial Effects of Bacterial Cellulose Produced by...
I hope this can help your research.
Thank you very much. A follow up question would be can this method work with Balb/C mice?
I appreciate your input.
Dear Muhammed Zidan Khan
It depends on what you want to accomplish with your research.
BALB/c Nude
It was bred by CR Japan (CRJ) by BALB/cABom-nu and BALB/cAnNCrj-nu cross and back cross. In 1985, CR introduced CAnN.Cg-Foxn1nu/Crl pure-line pregnant female mice from CRJ. This strain is an inbred strain and confirmed by genetic monitoring as BALB/c nude. This animal lacks a thymus, cannot produce T cells, and is, therefore, immune deficient.
It's not the mouse with hyperglycemia, so you must choose yourself.
Chou-Yi Hsu I understand. The purpose of our study is the development of an experimentally produced, non-genetic, novel mouse model capable of manifesting T2D complications after sustained and prolonged hyperglycaemia. Ideally, the hyperglycaemic condition should be the primary driver of the development of complications within the model, so I wanted to know what the best dosage would be for BALB/c mice to induce diabetes and sustain hyperglycaemia without having a fatal effect on the mice.
Hope this adds the context you were looking for.
Dear Muhammed Zidan Khan
If that is so, I have found some research for you as a reference.
Jiang, P., Zheng, W., Sun, X., Jiang, G., Wu, S., Xu, Y., ... & Ai, C. (2021). Sulfated polysaccharides from Undaria pinnatifida improved high fat diet-induced metabolic syndrome, gut microbiota dysbiosis and inflammation in BALB/c mice. International journal of biological macromolecules, 167, 1587-1597.
Nopparat, J., Nualla‑Ong, A., & Phongdara, A. (2020). Treatment with Pluchea indica (L.) Less. leaf ethanol extract alleviates liver injury in multiple low‑dose streptozotocin‑induced diabetic BALB/c mice. Experimental and Therapeutic Medicine, 20(2), 1385-1396.
I hope this can help your research.
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