My research aims to produce a novel mouse model for T2DM and I have two dosage techniques to administer STZ in C57BL/6 mice: single high dose (SHD) and multiple low dose (MLD). Within these groups, in my study design I have divided my animals into three subgroups:
- SHD: 75 mg/kg, 100 mg/kg, and 150 mg/kg
- MLD: 40 mg/kg, 50 mg/kg, and 60 mg/kg, each for 5 days.
When it came to the MLD groups, I basically sandwiched the standardised protocol provided by DiaComp of 50 mg/kg dose of STZ with a dose 10 mg/kg higher and lower. However, the ethics committee is requesting a substantiation for this and thus I would like to know if what I did is correct, and if there's literature to back this up, or if I should change my MLD dosage groups altogether. Please assist. Bear in mind, the model aims to assess the major microvascular complications, and thus the diabetic condition should mimic the later stages of the disease.
Thank you.
Hi Dr. Khan,
May these two review papers will help you. See the author's major idea and the summary of literature evidence in this field (according to the animal's type), and read the references used by them.
Gvazava IG, Karimova MV, Vasiliev AV, Vorotelyak EA. Type 2 Diabetes Mellitus: Pathogenic Features and Experimental Models in Rodents. Acta Naturae. 2022 Jul-Sep;14(3):57-68. doi: 10.32607/actanaturae.11751.
Gheibi S, Kashfi K, Ghasemi A. A practical guide for induction of type-2 diabetes in rat: Incorporating a high-fat diet and streptozotocin. Biomed Pharmacother. 2017 Nov;95:605-613. doi: 10.1016/j.biopha.2017.08.098.
Thank you very much Agnes Martins
By the way, I'm still doing my MSc, so I'm not Dr. Khan yet, but I do like the sound of it :)
Hi Khan,
I'm sharing a paper with you that discusses what you need and points out that there are different variations in the induction of experimental diabetes in animal models between laboratories. My recommendation is that you conduct assays with at least three doses, for example, 50, 75, 100 mg/kg of STZ, and evaluate the glucose levels in your mices. This will allow you to observe the behavior and dynamics, and then determine which model best suits your needs. Also, please consider whether you want to induce type 1 or type 2 diabetes. Just administering STZ will resemble type 1, but if you add diet or nicotinamide, it will resemble type 2 diabetes.
Best regards, Jorge Armando
Article Streptozotocin as a tool for induction of rat models of diab...
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