I have isolated PBMC from human blood and have set up an MLR for 5 days. I have then stained with CFSE and the cells have not proliferated.
Any help would be appreciated
Kais Khudhair al Hadrawi
MHC incompatibility, where differences in major histocompatibility complex (MHC) molecules between individuals lead to recognition as foreign or incompatible. The presence of immunosuppressive factors, either naturally produced or added to the culture environment, inhibits PBMC proliferation. Tolerance induction, where prior exposure to antigens from the other individual induces immune tolerance, resulting in a lack of proliferative response. The activation state of PBMCs before the MLR can influence their proliferative capacity, with already activated or exhausted cells exhibiting reduced potential. Poor cell viability or improper culture conditions affecting PBMC proliferation, such as inadequate nutrients or excessive cell density. Technical issues in experimental techniques, such as variations in cell isolation methods or culture conditions.
The lack of peripheral blood mononuclear cell (PBMC) proliferation in a mixed lymphocyte reaction (MLR) can be due to various reasons:
Addressing these factors requires careful consideration of immune recognition, tolerance mechanisms, environmental conditions, and experimental techniques to optimize MLR outcomes.
1 votes
1 thanks
More Sanskriti Agarwal's questions See All
Similar questions and discussions