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I have isolated PBMC from human blood and have set up an MLR for 5 days. I have then stained with CFSE and the cells have not proliferated. Any help would be appreciated
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Hi, I am doing a mixed lymphocyte reaction with isolated PBMCs. To assess proliferation I have tried using CFSE, however, I can only see one peak after day 5 and not multiple. Has anyone faced a...
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