It is an new ES cell line. They grow perfectly on MEFs but when replated on gelatin-coated dishes (or matrigel) after one day they start to die. We have changed all component of media...
Hello
Look at these links
Hope the help
https://www.encodeproject.org/documents/a1578b18-9712-4369-bb44-d3a9c7096541/@@download/attachment/Care%20and%20Culture%20of%20mES%20cells.pdf
https://catalog.coriell.org/0/Sections/Collections/NIA/nia_protocol_mesc.pdf
Thanks Saleh but I don't see any links
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