Hello all,
I am try to grow LNCaP cells on BD Matrigel to perform some invasion and migration assays. However, my cells gets clumped as u can see in the attached in pic (they look fine on uncoated plates/wells) . Matrigel was diluted 1:100 and allowed to solidify for 3 hours in 370C incubator.
Any insights, tips or hints will be much appreciated.
Thank you
Shreyas