I am carrying out cell-free DNA extraction using an in-house buffer system and magnetic nanoparticles. My qubit readings and tape station data look good. But upon using some plasma samples (2 out of 10)my elutes turn translucent. I am eluting with 50 uL of 10mM Tris Cl pH 8.7. I would like to know if anyone using magnetic beads are facing the same issue.
It would also be helpful if I could know about a certain property of plasma that might interfere in cfDNA extraction which results in the tinting of elutes.
Also, does storing blood samples in streak tube cause any affect in cell free DNA extraction?
Kindly help me out from this dilemma.
Awaiting your insightful responses.