Gayathri Subramanian

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Questions related from Gayathri Subramanian

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

29 July 2024 3,852 0 View

Why are my cell-free DNA elutes translucent after cfDNA extraction using plasma samples?

I am carrying out cell-free DNA extraction using an in-house buffer system and magnetic nanoparticles. My qubit readings and tape station data look good. But upon using some plasma samples (2 out...

12 January 2024 9,451 0 View

Upon using DMSO and betaine the bands in my NTC and my sample look different upon doing LAMP. What does that indicate?

I am trying to sort out this primer dimer (in NTC) issue for a very long time. After many of my previous trials in which NTC and my sample bands looked same, only in this trial the NTC and my...

30 May 2019 7,073 2 View

On working with LAMP how do we detemine the optimum temperature for the LAMP reaction?

When I did a PCR reaction using the template DNA of mosquito using the outer primers F3 and B3 I found my target region of interest amplifying at an annealing temperature of 55 degrees. But when...

22 March 2019 4,449 7 View

What are the drawbacks of using Strand Displacement Amplification PCR in the case of molecular diagnostics?

Isothermal amplification strategies are used for point of care disease diagnostics. I would like to know if SDA based amplification strategies would have any significant disadvantages.

14 January 2019 1,695 0 View

I am working on Synechosystis PCC 6803. I have to give salt stress to my microorganism. I am using BG-11 media. How do I add salt to my culture media?

Since BG-11 media has no NaCl content in it, I might have to add NaCl separately. If I add NaCl externally, what concentration of NaCl should I consider as my control? Kindly guide me regarding...

16 September 2018 6,201 5 View

I am working on Synechosystis PCC 6803. I have to give salt stress to my microorganism. I am using BG-11 media. How do I add salt to my culture media?

Since BG-11 media has no NaCl content in it, I might have to add NaCl separately. If I add NaCl externally, what concentration of NaCl should I consider as my control? Kindly guide me regarding...

16 September 2018 6,156 2 View

How should I select various salt concentrations while designing an experiment to study cyanobacterial photosynthesis under salt stress?

I am planning to design an experiment to study PS1 and PS2 activity of cyanobacteria in response to salt stress. How do I select various NaCl concentration levels to study salt stress? Kindly help...

09 August 2018 5,476 6 View

How to get competent cells that gives maximum transformation efficiency?

The problem is before subculturing the inoculated broth had to be kept in the fridge since it's not possible to do the experiment after 12 hrs. Also it is taking more time to reach log phase so we...

12 November 2017 9,137 1 View