What are typical Cu sources causing Cu adducts in ESI MS?

Hi everybody, currently I observe quite frequently Cu-adducts on my ESI high resolution MS, especially when analyzing ligands. There was no Cu used for the synthesis, so it must come from the...

21 October 2020 5,630 5 View

Which is the Risk of Long QT and Sudden Cardiac Death by Drugs Used to treat COVID-19?

Numerous drugs are used these days without in-depth evaluation of efficacy and safety for the treatment of life-threatening complications of COVID-19. Among these, an important number are known...

26 March 2020 816 17 View

Which pharmacological modulation of immune response to SARS COV-2 could reduce the incidence of acute respiratory distress syndrome?

Differences in immune responses to emerging corona viruses and the difficulties of developping vaccines contribute to a surge in ARDS in vulnerable populations, leading to acute shortages and...

16 March 2020 3,520 26 View

Does anyone know a freeware tool that can align exon and intron sequences to a template DNA sequence?

I am seaqrching for a tool that can align exon and intron sequences to a template DNA sequence without disrupting any of the short sequences. I remember that DNAStar was able to do this. Does...

12 December 2019 8,902 3 View

Does anyone have any early World History LGBT secondary sources that are non-western?

I am looking to integrate unheard voices into the survey class, before 1500. If you have secondary sources that you are willing to share please let me know.

22 October 2019 1,233 4 View

Taxonomy of Data Sources?

Can anyone suggest a reference that deals with a decent, contemporary taxonomy of data sources? There is the old taxonomy by Cattell: Q-Data (= Questionnaire Data), T-Data (= Test-Data), and...

22 October 2019 8,429 1 View

Why is the monoisotopic peak sometimes slightly different to the calculated m/z?

Hi, I just had a look on a recent analysis (on C26H19F4N2O4Se+) and was wondering why the simulated monoisotopic peak of the molecular ion (579.0441) is slightly differnt to the calculated m/z...

26 March 2019 7,774 12 View

Are there free online tools for the visualization of gene set (GO-) enrichment analysis results?

Gene set enrichment analyses usually end up with lists of enriched categories associated with a p-value or some othewr measure of reliability. I am looking for free visualization tools such as...

07 January 2019 9,701 1 View

Which staining method to use to quantify low level nerv damage?

02 April 2017 4,332 3 View

Does anyone have a transfection protocol for the NSCLC cell line PC9?

I use HiPerFect from Quiagen. I transfect microRNAs which lead to a reduction of their targets in other cell lines like HCC4011, HCC827 and A549 and siGLO as a control. But I can't detect...

17 February 2017 3,919 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How many ug should I choose as the protein amount when doing western blot in liver tissue?

25 January 2021 794 3 View

Tick-borne encephalitis (TBE) incidence in the Czech Republic in recent years?

Dear Sir or Madam, i'm a medical student. In range of school lesson i have to research about TBE (see topic) in the country of Czech Republic. I would be pleased if you could give me a hand on...

24 January 2021 5,984 4 View