Hi, my lab assistant performed an SDS-PAGE electrophoresis as follows: Load order: well 1 corresponds to the standard, well 2-3-4 correspond to BSA proteins, Cytochrome c and Wheat germ acid phosphatase which are 5æl of each protein plus 5æl of water and 5æl of loading buffer were loaded.
Wells 5-6-7 also correspond to the same order of proteins but with 10 μl of each plus 5 μl of loading buffer. (total volume to be charged 20 ul)
Therefore, as you can see, an excess of protein was added and therefore do not consider the concentration of each protein (ug / ul)
The gel used was 10% as it leaves the guide.
I recommend that you discuss about the appropriate range to detect by comassie blue on polyacrylamide gels. They determine the MP of each protein and contrast it with the literature and can also discuss why a large number of bands are seen in the lanes that are presented. Also advantages or disadvantages of loading an excess of proteins.
Thank U!
In lane number 7? Why are bands seen in series on SDS-PAGE electrophoresis?
Hi Kari: As CB binds different proteins/polypeptides by a combination of hydrophobic interactions and heteropolar bonding with basic amino acids ( PMID: 18327568 ), it's staining intensity does not reflect the same quantities for various proteins. In terms of the large number of bands, they may represent polymers or aggregates of one protein or its chaperones such as alpha2-macroglobulin in plasma proteins (e.g. BSA), or impurities of a "purified protein" (contaminants, e.g., wheat germ acid phosphatase). Hope it partly answered your questions.
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