hybrid capture PCR can handle more than 600 genes as a panel, but anchored multiplex PCR can only have no more than 100
Article Next Generation Sequencing for Gene Fusion Analysis in Lung ...
Article NGS-PrimerPlex: High-throughput primer design for multiplex ...
When I measured the cyclic voltammogram of palladium in the pbs solution, two reduction peaks appeared. This did not match the literature I saw. Why? And how can I find the possible reaction...
20 February 2021 2,486 3 View
Hi, everybody. I want to know the primers for acetylcholinesterase in guinea pigs when we do RT-PCR
05 November 2020 9,085 2 View
Hi guys, I am trying to understand if the water bottom type does not change, its reflectance at a certain wavelength changes or not?
15 October 2020 6,297 3 View
Among gravel, sands, silt and clay, these grains have different sizes. Except their original materials, do they appear different colours due to different abilities of reflection?
04 November 2019 1,279 5 View
Hi, I would like to process my data in Matlab (I have to process them in Matlab). Is it possible to convert the DN value (band 2) of a sentinel 2 imagery to a matrix, which means each cell...
16 September 2019 6,267 3 View
Hi guys. I wanna combine the scatters and curves in the same graph. However, I used spline function in curves. The graph twisted a lot. The expected graph is the...
28 August 2019 6,684 1 View
Dear all, I'd like to calculate the intersystem crossing rate using Dalton package, for example, from S1 to T1. Now I can only get the spin orbit coupling matrix element (SOCME), but don't know...
21 August 2019 6,595 4 View
Can these residuals be regarded heteroscadastic? I have tested the residuals are normally distributed.
23 May 2019 5,173 4 View
hello, every researcher, If I want to study the relationship between an iv and a dv, and the moderation effect of gender, will the analytic plan shown below be appropriate? 1. MANOVA and Post...
26 November 2018 5,402 3 View
Hi everyone! I'm new to the world of flow cytometry, I want to count the T cells in the rat liver, After getting the monocytes from the liver, I stained the CD3, then first I gate the monocytes...
26 July 2017 2,833 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
02 March 2021 7,082 3 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...
01 March 2021 6,200 3 View