10 March 2022 1 7K Report

hybrid capture PCR can handle more than 600 genes as a panel, but anchored multiplex PCR can only have no more than 100

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How to batch extract texts that cite your papers?

Suppose I'm not only interested who and which article cited my paper, but also what's the specific text they mentioned that cited my article. Is there a quick way to batch extract such information?

16 July 2024 9,702 1 View

Are there any people using biolegend anti- mouse CCR2-BV785?

Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...

03 July 2024 3,969 4 View

Is it hard to stain CCR2 for flow cytometry?

Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...

08 June 2024 7,315 5 View

How to use microbes to remediate soil contamination quickiy, efficiently and cheaply?

Be curious and want to learn something from cases

20 May 2024 5,073 4 View

Volcano plots - why there were some long gaps inside the volcano without any dots?

Hello, everyone! I got a very weird shape of volcano plot with R. It doesn't have a typical shape and I want to figure out the reasons behind this problem. In my plot, I found there were some...

10 May 2024 3,339 2 View

How to do tumor mouse model properly?

Hi guys, I'm working on the tumor mouse model. In general, I prepared tumor cells and injected cells into the flank of mice subcutaneously. After around 3 weeks, I found, in the same group,...

29 February 2024 9,034 3 View

Quality of bacterial genomic DNA obtained by different extraction methods?

Hello, I would like to ask if there is a significant difference in the size of genomic DNA obtained by different bacterial genome extraction methods (such as mild enzymatic lysis or violent...

15 January 2024 2,916 1 View

Understanding some strange crystal morphology?

Recently obtained some nice bulk crystals of layered perovskites. These crystals are usually terminated with acute trapezoidal end facets making them look like gold bars. but when they are grown...

13 January 2024 8,423 6 View

Why does the threshold rate change in BD device?

Hi, I'm new to flow cytometry. Basically, I measured immune cells in the heart using the BD device. And I found sometimes the range of threshold rate was not similar among samples. For example, I...

21 November 2023 941 1 View

How to count cells after tumor immune cells isolation?

Hi, I'm working on the immunity of tumor. I injected tumor cells into the mice subcutaneously. After harvesting the tumor, I used percoll and isolated immune cells. Then I added trypan blue and...

20 November 2023 7,668 4 View

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