Hi,
I'm working on the immunity of tumor. I injected tumor cells into the mice subcutaneously. After harvesting the tumor, I used percoll and isolated immune cells. Then I added trypan blue and counted cells using a cell counting chamber before flow cytometry staining.
I found in most of the papers, they just said "Count cells for staining". But they didn't say count which kind of cells. Because I saw there were different type of cells in the microscope.
Do I need to count all living cells, no matter how different the shape they are. Or do I just count one specific cell?
Many thanks.
If this is for flow cytometry staining, count all the cells! Disregard any difference in cell type.
Louis-Charles Béland Yes, for flow cytometry staining. Thank you so much.
Definitely agree that you need to count all cells and use flow to quantify populations. We recently integrated cell counting beads into our flow assays which have helped streamline analysis. I believe we use the Thermo Fisher Count Bright Plus beads.