Hello, I would like to ask if there is a significant difference in the size of genomic DNA obtained by different bacterial genome extraction methods (such as mild enzymatic lysis or violent physical disruption methods)? Recently, I used a glass bead disruption method to extract genomic DNA from a strain of Streptomyces. The final DNA obtained after agarose electrophoresis analysis always had a slightly lower band than the 23 kb marker band. Is this normal? If I use a gentle genomic DNA extraction method, can I obtain more complete genomic DNA? Thank you for your attention and reply.
Yes, the method used for bacterial genomic DNA extraction can significantly impact the size and integrity of the DNA obtained. Different methods, such as mild enzymatic lysis versus physical disruption (like glass bead beating), have varying effects on DNA quality and quantity. Here are some key points to consider:
In summary, switching to a gentler genomic DNA extraction method may help you obtain more intact genomic DNA, especially for applications that require high-molecular-weight DNA. However, the efficiency of cell lysis and the overall yield might be different, so you might need to optimize the process for your specific bacterial strain.
I hope that made sense to you.
Feel free to ask if anything was unclear.
Best
Dr. Pri
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