Prabodh Kumar

3 Questions 32 Answers 0 Followers

Questions related from Prabodh Kumar

What is the optimum laser strength one must use during confocal microscopy?

I am dealing with a weak dye and a weak laser. I have previously read that the laser strength should not be more than 50%. Is it a rule or just a normal guideline? I cannot see the signal clearly...

06 June 2014 694 15 View

Can anyone help with super fast fluorescence quenching?

I am using CY-3 dye in my Confocal experiment. The florescence is relatively weak and it gets quenched almost instantly during scanning and acquiring the image. I m not sure if the problem is with...

06 June 2014 2,521 6 View

If a HL-1 cardiomyocyte culture is not contracting is it normal?

I have recently established an HL-1 cardiomyocyte cell line from a frozen aliquot. I don't see the cells contract in the culture. I have already passaged them 4-5 times. I want to study calcium...

05 May 2014 6,107 6 View