Is it possible to get cuvettes for the Bausch and Lomb spectronic 600?

We have an old Bausch and Lomb spectronic 600 (I think that's the right model number) spectrophotometer. Not exactly cutting edge technology, but it's simple and robust and adequate for taking...

31 December 2019 9,618 1 View

How to measure bacterial growth by protein measurement?

We are studying with plastic-degrading microbes. Although we are getting turbidity increases, we need a different way of measuring bacterial growth as the powdered plastic contributes to the...

09 October 2019 4,393 4 View

How to sequence short PCR products?

We are currently using Sanger sequencing to sequence a 130 bp PCR product and it's not working too well. My colleagues tell me that it is fairly usual to get poor quality sequence for the first...

10 November 2018 3,061 7 View

How can we remove the slime capsule from microalgae?

We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...

09 October 2016 7,882 3 View

Does anyone have stocks of lambda transducing phages carrying the mre and or mrd genes of E. coli?

Read 1 answer by scientists to the question asked by Andrew Jenkins on Dec 10, 2014

11 December 2014 6,502 1 View

Can anyone offer advice on electroporating clinical strains of E. coli?

We are having trouble transforming plasmids into clinical strains of E. coli by electroporation. We use a 1500 V pulse and standard electroporation buffer and follow the protocol that comes with...

01 February 2014 7,791 3 View

Is it possible to amplify a plasmid that codes for chloramphenicol resistance?

We are working with a couple of plasmids which are rather difficult to isolate in satisfactory quantities. We tried chloramphenicol amplification on one of them and this made a huge difference and...

01 February 2014 6,078 5 View

Bacterial genetics: Does anyone have a reference for cross-streak mating?

Does anyone have a reference for this method. It's a very old technique - probably 40 years or more, but I can't find a book or a paper that describes it or refers to it.

11 December 2013 844 2 View

Is it possible to attach polyacrylamide gels to a membrane support?

I seem to remember some sales literature on such a product, but that was years ago and it doesn't seem to have caught on.

05 June 2013 420 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View