do you mean to do electroblotting and mass spectrometric analyses of proteins by transferring your protein from PAGE gels to a membrane ???

if that is what you mean this publication might be useful

http://www.ncbi.nlm.nih.gov/pubmed/21848805

Do you mean gel drying (natural dry)?

Here is an idea. Use a standard agrose gel apparatus and pouring frame. Somehow attach the membrane to the bottom of the pouring frame. Mix your acrylamide. Degas using a vacuum and a too large stopper connected to the vacuum hose. Pour the acrylamide into the frame. Put comb into position. Place the frame into a Tupperware container that has two ports with hose connectors. Flush with nitrogen, methane or argon. It wiil make a great gel and the membrane will be on the bottom. Run in the agrose apparatus and then run down to get to the membrane. I make this type of gel often, but without the membrane. Good luck.

I don't know about membranes (except of course for blotting), but there was a plastic film I once used for very flimsy gels - if you're interested I could try to dig up the name of it. As I recall you included it between the plates when pouring the gel, which adhered to it pretty much permanently. If flimsy gels are the issue, we also had good luck with Duracryl.

They could tell me a simple protocol of wester-blot from kidney extract in non-reducing conditions. Regards

Hi Guys, and thanks for all the suggestions. Pretty much everyone thought I was talking about doing a western blot, so I guess I didn't express myself too clearly. Barbara MacGregor did get my point - I'm looking for some kind of backing film to make flimsy gels easier to handle. Thanks, Barbara, it'd be great if you could dig up the name of that plastic film.

Please see the inserted publication below. It might be useful to know that are some scanners which may do stain-free for gels and the gel handle is minim.

Top Curr Chem. 2013;331:37-54. doi: 10.1007/128_2012_321.

Application of MALDI-TOF-mass spectrometry to proteome analysis using stain-free gel electrophoresis.

Susnea I, Bernevic B, Wicke M, Ma L, Liu S, Schellander K, Przybylski M.

Good Luck !

Hi Andrew,

Wow, found it in the first notebook I checked. Unheard of. It's called GelBond PAG film, that should be enough to find it (if it's still made). Also look for Duracryl, it made a big difference.

Barbara

Thanks Barbara. That's the name I was trying to remember. :).

And I'll check out the Duracryl.