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Questions related from Andrew Jenkins
We have an old Bausch and Lomb spectronic 600 (I think that's the right model number) spectrophotometer. Not exactly cutting edge technology, but it's simple and robust and adequate for taking...
01 January 2020 9,713 1 View
Does anyone know who now supplies LUX (Light upon extension) primers. These used to be available from Invitrogen, now a part of Thermo-Fisher, but Thermo-Fisher have discontinued the product and...
01 January 2020 6,395 0 View
We are studying with plastic-degrading microbes. Although we are getting turbidity increases, we need a different way of measuring bacterial growth as the powdered plastic contributes to the...
10 October 2019 4,492 4 View
We are currently using Sanger sequencing to sequence a 130 bp PCR product and it's not working too well. My colleagues tell me that it is fairly usual to get poor quality sequence for the first...
11 November 2018 3,176 7 View
We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...
10 October 2016 7,961 3 View
Read 1 answer by scientists to the question asked by Andrew Jenkins on Dec 10, 2014
12 December 2014 6,601 1 View
We are working with a couple of plasmids which are rather difficult to isolate in satisfactory quantities. We tried chloramphenicol amplification on one of them and this made a huge difference and...
02 February 2014 6,174 5 View
We are having trouble transforming plasmids into clinical strains of E. coli by electroporation. We use a 1500 V pulse and standard electroporation buffer and follow the protocol that comes with...
02 February 2014 7,889 3 View
Does anyone have a reference for this method. It's a very old technique - probably 40 years or more, but I can't find a book or a paper that describes it or refers to it.
12 December 2013 943 2 View
I seem to remember some sales literature on such a product, but that was years ago and it doesn't seem to have caught on.
06 June 2013 514 9 View
