Sorry, we don have one
Does anyone know who now supplies LUX (Light upon extension) primers. These used to be available from Invitrogen, now a part of Thermo-Fisher, but Thermo-Fisher have discontinued the product and...
31 December 2019 6,431 0 View
We have an old Bausch and Lomb spectronic 600 (I think that's the right model number) spectrophotometer. Not exactly cutting edge technology, but it's simple and robust and adequate for taking...
31 December 2019 9,750 1 View
We are studying with plastic-degrading microbes. Although we are getting turbidity increases, we need a different way of measuring bacterial growth as the powdered plastic contributes to the...
09 October 2019 4,523 4 View
We are currently using Sanger sequencing to sequence a 130 bp PCR product and it's not working too well. My colleagues tell me that it is fairly usual to get poor quality sequence for the first...
10 November 2018 3,214 7 View
We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...
09 October 2016 8,000 3 View
We are having trouble transforming plasmids into clinical strains of E. coli by electroporation. We use a 1500 V pulse and standard electroporation buffer and follow the protocol that comes with...
01 February 2014 7,931 3 View
We are working with a couple of plasmids which are rather difficult to isolate in satisfactory quantities. We tried chloramphenicol amplification on one of them and this made a huge difference and...
01 February 2014 6,212 5 View
Does anyone have a reference for this method. It's a very old technique - probably 40 years or more, but I can't find a book or a paper that describes it or refers to it.
11 December 2013 984 2 View
I seem to remember some sales literature on such a product, but that was years ago and it doesn't seem to have caught on.
05 June 2013 555 9 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
