Sorry, we don have one
Does anyone know who now supplies LUX (Light upon extension) primers. These used to be available from Invitrogen, now a part of Thermo-Fisher, but Thermo-Fisher have discontinued the product and...
31 December 2019 6,304 0 View
We have an old Bausch and Lomb spectronic 600 (I think that's the right model number) spectrophotometer. Not exactly cutting edge technology, but it's simple and robust and adequate for taking...
31 December 2019 9,618 1 View
We are studying with plastic-degrading microbes. Although we are getting turbidity increases, we need a different way of measuring bacterial growth as the powdered plastic contributes to the...
09 October 2019 4,393 4 View
We are currently using Sanger sequencing to sequence a 130 bp PCR product and it's not working too well. My colleagues tell me that it is fairly usual to get poor quality sequence for the first...
10 November 2018 3,061 7 View
We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...
09 October 2016 7,882 3 View
We are having trouble transforming plasmids into clinical strains of E. coli by electroporation. We use a 1500 V pulse and standard electroporation buffer and follow the protocol that comes with...
01 February 2014 7,791 3 View
We are working with a couple of plasmids which are rather difficult to isolate in satisfactory quantities. We tried chloramphenicol amplification on one of them and this made a huge difference and...
01 February 2014 6,078 5 View
Does anyone have a reference for this method. It's a very old technique - probably 40 years or more, but I can't find a book or a paper that describes it or refers to it.
11 December 2013 844 2 View
I seem to remember some sales literature on such a product, but that was years ago and it doesn't seem to have caught on.
05 June 2013 420 9 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
