We are doing enzyme kinetics with enzyme immobilized on nanostructure, we found that the enzyme activity is more efficient than its optimum experimental condition.
Yes, it may happen as immobilization on nanostructures makes enzyme more stable (highly surface area to volume ratio) and more active or efficient (possibly by exposing active sites towards substrate).
Regards
Abhishek Sharma
Are you asking whether it is reasonable for the activity of the immobilized enzyme to be higher than the activity of the enzyme free in solution? If so, my question is, how do you determine the amount of immobilized enzyme in the reaction?
Yes, it may happen as immobilization on nanostructures makes enzyme more stable (highly surface area to volume ratio) and more active or efficient (possibly by exposing active sites towards substrate).
Regards
Abhishek Sharma
The optimum condition can be affected because of mode of attachment of protein onto nanostructure. there might be increase in thermostability and optimum temperature or stability after attachment to the nanostructure.
please find these piblications for your reference.
https://www.researchgate.net/publication/325356717_High_throughput_synthesis_of_ethyl_pyruvate_by_employing_superparamagnetic_iron_nanoparticles-bound_esterase
Article Improved catalytic properties of a serine hydroxymethyl tran...
https://www.researchgate.net/publication/324689266_Synthesis_and_characterization_of_cross_linked_enzyme_aggregates_of_serine_hydroxyl_methyltransferase_from_Idiomerina_leihiensis
https://www.researchgate.net/publication/320988987_SnO2_hollow_nanotubes_A_novel_and_efficient_support_matrix_for_enzyme_immobilization
Article Immobilization of a novel cold active esterase onto Fe3O4∼ce...
Chapter Nano-particles in biochemical engineering: Synthesis and applications
https://www.researchgate.net/publication/257908646_Effective_immobilisation_of_lipase_to_enhance_esterification_potential_and_reusability
https://www.researchgate.net/publication/298208863_Catalytic_Potential_of_a_Nitrocellulose_MembraneImmobilized_Lipase_in_Aqueous_and_Organic_Media
Article Enzymatic Synthesis of Butyl Ferulate by Silica-Immobilized ...
Thank You @ Dr Adam B Shapiro, Dr Abhishek Sharma, Dr Ashok Kumar Nadda Sir.
The physio-chemical properties of the natural enzymes will be different when nano structures particularly metals /coenzymes are bound to the protein enzymes
Green and Sustainable Chemistry, 2014, 4, 15-19
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