What is protein corona (Hard/Soft), and how it is from?

More Bipin S Khade's questions See All

What is the importance of substrate immobilization on matrix support in enzymatic reaction?

In enzymatic reaction generally researchers immobilized enzyme on matrix support for increase/decrease in catalytic performance of enzyme, is there any possible explanation for substrate...

05 June 2018 9,035 0 View

How can I determined average size of nanoparticles from TEM data?

We synthesize silver nanoparticles, using biological methods, in TEM image there are variety of SNPs size like, 13 nm, 16.15 nm, 20 nm, 16.39 nm.

05 June 2018 778 3 View

How to synthesize porous ZnO nanosheet?

Synthesis of porous ZnO nanosheet for industrial applications.

04 May 2018 6,831 4 View

If quenchers acts as enhancers in fluorescence intensity, what is the possible explanation?

In fluorescence spectrometer experiment when quencher (Not a fluorophore) is added the fluorescence intensity increases upon time duration.

04 May 2018 6,676 4 View

While enzyme immobilized with nanostructure does optimum experimental condition is affected for enzyme?

We are doing enzyme kinetics with enzyme immobilized on nanostructure, we found that the enzyme activity is more efficient than its optimum experimental condition.

04 May 2018 9,100 6 View

While synthesis of silver nanoparticles, absorbance is increases with varying AgNO3 (Salt) Con'c. but SPR is same, what is the possible explanation?

We synthesize silver nanoparticles using silver nitrate salts, while synthesis of SNPs absorbance increases but Surface Plasmon Resonance (SPR) peak was same but size of SNPs changes, is there...

04 May 2018 7,617 3 View

Synthesis of nanostructure material like silver and gold using protein molecule, has more stability, what is the possible phenomenon?

Protein play vital role in synthesis of various nanomaterials, synthesize nanomaterials having more stability and unique SPR peak as compare other methods. Cysteine amino acid may involve for...

04 May 2018 9,859 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why am I getting "shark finn"-like signal?

My collegue and I are having hard time to understand what is happening. Basically we have a tapping device that work with Arduino and a pneumatic pump. The pump allows the tapping of two...

04 August 2024 4,662 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Sharafaldin Al-Musawi

i recommend you to study following link:

Article Protein corona: A new approach for nanomedicine design

Hugh J Byrne

Check also the work of Marco Monopoli, e.g.

Article Formation and Characterization of the Nanoparticle–Protein Corona

Bipin S Khade

Thank You @Dr Sharafaldin Al-Musawi, Dr. Hugh J Byrne Sir