What do you think makes an effective classroom-instruction?

class management ;interaction between teachers and students;

16 November 2020 5,301 4 View

Which tools do you use for Systems Engineering?

Using a perfect toolchain for modeling every aspect of a system is possibly a dream. But perhaps one of you can recommend a tool, that supports most systems engineering tasks.

06 February 2020 2,909 3 View

Suggested packages/programs for automated detection of species-specific bird vocalizations?

Does anyone have suggestions for a user-friendly, open source, automated process for identifying species-specific bird vocalizations from audio recordings? I'm currently teaching myself to use...

04 February 2020 2,362 3 View

Can anyone recommend some good resource for learning identification of North American freshwater snails?

I work primarily in Arkansas. I know there's no field guide or anything that would be the snail equivalent of Merritt and Cummins Introduction to Aquatic Insects, but some regional resources for...

26 April 2018 9,141 1 View

Will a luciferase promoter activity assay work if the test cells don't endogenously express the gene of interest?

I am currently working up a set of experiments to determine the signaling pathways regulating transcriptional control of our gene of interest. We already have full length and truncated promoter...

01 June 2016 9,506 1 View

What is the decay curve of a plasmid in the nucleus of a *quiescent* cell, or of protein expression therefrom?

The half-life of a plasmid in cells in culture is fairly brief, but a lot of that is presumably because of dilution due to cell division. What happens when a plasmid has achieved entry into the...

07 March 2016 1,784 1 View

How do I find references from Cryst. Struct. Commun. from the time before this term was attached to Acta Crystallographica C?

I know such publications exist from references to my own work but can not find the original articles.

06 December 2013 6,199 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View