01 March 2013 1 4K Report

Want to know the ups and downs of two feeder layer culture systems for iPSCs.

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Thematic analysis or grounded theory?

i am currently conducting a critical appraisal of a research article and the data analysis is explained as: interviews transcribed verbatim and participants were given the option to review and...

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What are the antimicrobial resistance patterns in the Philippines vs. global trends, especially in common antibiotics, and how do they address these?

Antimicrobial resistance (AMR) is a growing concern globally, particularly in third-world countries like the Philippines, where access to healthcare resources may be limited and antimicrobial...

08 April 2024 6,343 3 View

Genotype binary -1/0/1 convert?

I have hapmap or Plink format. I want genotype binary -1/0/1 convert I want to create an input file in BGLR or BWGS to use gBLUP. If you have an R package or any other good method, I'd love to...

08 April 2024 8,268 2 View

What econonometric method should I use if my dependent variable has a data that is collected every three years or have a gap/missing data??

I have been working on my paper where the only dependent variable that I can use, and is fitted based on my research objectives, is a data from a survey that is taken every 3 years. My other...

09 March 2024 9,417 5 View

What are students perspectives on school fights?

What do students think are the reasons for school fights

26 February 2024 4,633 5 View

Fluorescent nanobodies that target GFP?

I'm looking for anti GFP fluorescent nanobodies conjugated with Alexa 647 (or equivalent) for immunostaining of samples with GFP in order to do dSTORM imaging. Does anyone know of any commercially...

20 November 2023 4,236 0 View

Best staining protocol for live neuron culture calcium staining?

I am working on a protocol to measure calcium flux by synaptic signaling in live mouse neuron culture. I am using Fluo-4 AM for my stain, which I have seen others use, but it is not showing any...

31 January 2023 7,305 4 View

Fluo-4 AM signal decay?

Hi all, I'm staining live neurons with Fluo-4 AM esters (Thermofisher cat# F14201) and seem to be having some issues when capturing images. With video recording with continuous excitation, there...

02 November 2022 2,559 0 View

How Do You Make a Flathead ("Inhibits") Signaling Arrow for PowerPoint?

That's the question. PowerPoint has "Shapes" for arrows of various types, but they're all of the pointed variety, and neither BioRender nor Servier Medical Art seems to have one.

06 May 2022 7,814 0 View

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