06 June 2015 0 5K Report

I am looking forward to monitor a reaction of conversion of phosphorylase b to a and vice versa.

More Sanjay Mishra's questions See All
Should we treat 'Aging' as a disease?

Medical Professionals, Biochemists, Molecular Biologists

06 July 2016 1,002 0 View

Similar to macromolecules like carbohydrates, protein and lipids, is it possible to identify an enzyme using a single step protocol?

If the source is known and we are interested in modifying the source quality by altering the enzymatic modulation.

04 May 2014 8,071 3 View

What are the major biochemical and biophysical techniques to isolate and purify a multienzyme complex?

Pyruvate dehydrogenase complex.

03 April 2013 4,290 0 View

How can a 'Protein Chemist' coordinate with an 'Enzymologist'?

Google

01 February 2013 9,800 2 View

As far as my experience is concerned, soluble enzymes are rather easier to immobilize. How can this be easily applicable to membrane bound enzymes?

Google

01 February 2013 705 0 View

How can team spirit be helpful in upgrading the level of research output?

How can team spirit be helpful in upgrading the level of research output?

31 December 2012 2,199 4 View

How can the knowledge of biochemistry be helpful in explaining and propagating 'Bioprocess and Fermentation Technology'?

How can the knowledge of biochemistry be helpful in explaining and propagating 'Bioprocess and Fermentation Technology'?

31 December 2012 2,511 4 View

Do data obtained from X-Ray Crystallography explore the studies concerning with molecular dynamics using computational biology ?

-

11 December 2012 3,006 8 View

Which one out of 'Impact Factor' and 'SCI' is better to explain the worth of a 'Research Paper'?

Google

11 December 2012 7,767 6 View

Why does the activity of enzymes decrease as they are diluted in aqueous solutions/mixtures?

Why does the activity of enzymes decrease as they are diluted in aqueous solutions/mixtures?

11 December 2012 2,929 7 View

Similar questions and discussions
How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close