Dear all,
If I have to collect blood from fish juveniles, what should be the needle size that I should be using. Kindly help.
Thanks in advance.
Hi Aparna,
A narrow insulin syringe should be the best option. However, if you need to use a larger or smaller instrument that might provide more options. You should be able to work out the best option once you decide on the best size to use,
Regards
Gordon
Ok, we use insulin siring for caudal vein, and can use 3, 5, or 10ml (Jai Ghosh) for heart.
To get blood samples from 30 g channel catfish, I used a pediatric vaccutainer (purple top with EDTA) and a 22 guage needle.
In our case we use insulin syringes fish with a minimum weight of 50 grams
Dear Aparna,
To withdraw blood from 20g fish the, options depend on the purpose of the blood sample (parameters to be analysed)
(a) 1 ml insulin syringe with nondetachable needle: If this is used, the possibility of blood cells being ruptured (haemolysis) while emptying the blood from the syringe is high (due to pressure of pushing the blood through a very narrow needle). This can be used if the analysis is to be performed on serum but not plasma/whole blood.
(b) 2 ml syringe with 24 or 25 guage needle: Only well skilled and experienced persons can handle this model to withdraw blood from 20g fish with ease. I had done many times, it gives the option to remove the needle and empty the blood sample without damaging the blood cells.
Hope this info might be useful.
All the best
Antony
Dear Dr. Antony,
Thank you very much for your response. With the 22 guage needle, I could not withdraw any blood from the fish. I will try and use the insulin syringe preferably with disposable needle, as suggested.
Dear Aparna,
Use 24 gauze 5ml dispovan. It will work. You need to ringe the syringe with EDTA solution then draw blood. I have done 100s of times.
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